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KMID : 0363620140350040036
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Journal of Korean Oriental Medicine
2014 Volume.35 No. 4 p.36 ~ p.51
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Classification of the Efficacy of Herbal Medicine Alterations in Neuronal Hypoxia Models through Analysis of Gene Expression
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Hwang Joo-Won
Shin Gil-Cho
Moon Il-Su
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Abstract
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Objectives: cDNA microarray is an effective method to snapshot gene expression. Functional clustering of gene expressions can identify herbal medicine mechanisms. Much microarray data is available for various herbal medicines. This study compares regulated genes with herbal medicines to evaluate the nature of the drugs.
Methods: Published microarray data were collected. Total RNAs were prepared from dissociated hippocampal dissociate cultures which were given hypoxic shock in the presence of each herbal medicine. Up- or downregulated genes higher than Global M value 0.5 were selected, clustered in functional groups, and compared with various herbal treatments.
Results: 1. Akt2 was upregulated by Acorus gramineus SOLAND, Arisaema amurense var. serratum NAKAI and Coptis chinensis FRANCH, and they belong to Araceae herb. 2. Nf-κb1, Cd5, Gnγ7 and Sgne1 were upregulated by Arisaema amurense var. serratum NAKAI, Coptis chinensis FRANCH and Rheum coreanum NAKAI. 3. Woohwangcheongsim-won, Sohaphyang-won and Scutellaria baicalensis GEORGI downregulated Scp2 and upregulated Tsc2. Woohwangcheongsim-won and Sohaphyang-won upregulated Hba1 and downregulated Myf6. 4. Sohaphyang-won and Scutellaria baicalensis GEORGI downregulated Slc12a1. 5. Woohwangcheongsim-won and Arisaema amurense var. serratum NAKAI upregulated Rarα, Woohwangcheongsim-won and Coptis chinensis FRANCH downregulated Rab5a and Pdgfrα, and Woohwangcheongsim-won and Rheum coreanum NAKAI upregulated Plcγ1 and downregulated Pla2g1b and Slc10a1.
Conclusions: By clustering microarray, genes are commonly identified to be either up- or downregulated. These results will provide new information to understand the efficacy of herbal medicines and to classify them at the molecular level.
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KEYWORD
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Hypoxia, apoptosis, gene expression, microarray
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